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IL8 polyclonal antibody

货号: BS7145

价格：￥ 1140

货期：现货（1-2个工作日）

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详细信息检测数据实验指南文献

No. :BS7145

Product Name :IL8 polyclonal antibody

Swiss-Prot :P10145

Host :Rabbit

Reactivity :Human, Rat

Applications :WB, IHC

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200

Background :The protein encoded by this gene is a member of the CXC chemokine family. This chemokine is one of the major mediators of the inflammatory response. This chemokine is secreted by several cell types. It functions as a chemoattractant, and is also a potent angiogenic factor. This gene is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. This gene and other ten members of the CXC chemokine gene family form a chemokine gene cluster in a region mapped to chromosome 4q.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :10KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :CXCL8;GCP-1;GCP1;IL8;LECT;LUCT;LYNAP;MDNCF;MONAP;NAF;NAP-1;NAP1

Immunogen :A synthetic peptide of human IL8(NP_000575.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of Recombinant Human IL-8 Protein, using IL8 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 1s.

Western blot analysis of extracts of Rat lung, using IL8 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 90s.

Immunohistochemistry of paraffin-embedded human appendix using IL8 Rabbit pAb at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Gene expression in human umbilical vein endothelial cells exposed to fine particulate matter: RNA sequencing analysis

JournalInternational Journal Of Environmental Health Research

ApplicationWB

IF3.411

SpeciesHuman

PMID34102927
Insights into the functional role of grass carp IL-8 in head kidney leukocytes: pro-inflammatory effects and signaling mechanisms

JournalJournal Of Fish Biology

ApplicationWB

IF2.051

SpeciesFish(Grass Carp)

PMID34716580
Therapeutic effects of shikonin on adjuvant-induced arthritis in rats and cellular inflammation, migration and invasion of rheumatoid fibroblast-like synoviocytes via blocking the activation of Wnt/β-catenin pathway

JournalPHYTOMEDICINE

ApplicationWB

IF6.656

SpeciesHuman

PMID37163904