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ASNA1 polyclonal antibody

货号: BS71411

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS71411

Product Name :ASNA1 polyclonal antibody

Swiss-Prot :O43681

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IF/ICC

Application_all :WB,1:200 - 1:2000|IF/ICC,1:50 - 1:200

Background :This gene represents the human homolog of the bacterial arsA gene, encoding the arsenite-stimulated ATPase component of the arsenite transporter responsible for resistance to arsenicals. This protein is also a central component of a transmembrane domain (TMD) recognition complex (TRC) that is involved in the post-translational delivery of tail-anchored (TA) proteins from the cytosol to the endoplasmic reticulum (ER). It recognizes and selectively binds the TMD of TA proteins in the cytosol, and delivers them to the ER for insertion.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :39kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :ASNA1;ARSA-I;ARSA1;ASNA-I;GET3;TRC40;hASNA-I

Immunogen :Recombinant fusion protein of human ASNA1(NP_004308.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using ASNA1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 90s.

Immunofluorescence analysis of HeLa cells using ASNA1 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using ASNA1 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.