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UBE2Q2 polyclonal antibody

货号: BS71401

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS71401

Product Name :UBE2Q2 polyclonal antibody

Swiss-Prot :Q8WVN8

Host :Rabbit

Reactivity :Human, Mouse

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:100

Background :Ube2Q2 is an E2 enzyme, which is part of the E1, E2, and E3 cascade responsible for ubiquitination of protein substrates. Ube2Q2 is thought to play a key role in regulating mitotic stages in cells. Its discovery was in connection with an arrest in the mitotic cell cycle. Inactivating this E2 prohibits the cell from continuing on past prophase. In cases of head and neck squamou cell carcinaoma, there is an elevated level of Ube2Q2 in the cancerous tissues. Also when the expression levels decreased, the cancerous cells were found to have a resistance to chemotherapeutic agents.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :43kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :UBE2Q2

Immunogen :Recombinant fusion protein of human UBE2Q2(NP_775740.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using UBE2Q2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 60s.