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RPE65 polyclonal antibody

货号: BS71345

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS71345

Product Name :RPE65 polyclonal antibody

Swiss-Prot :Q16518

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200

Background :This gene encodes a protein which is located in the retinal pigment epithelium and is involved in the production of 11-cis retinal and in visual pigment regeneration. There are two forms of this protein, a soluble form called sRPE65, and a palmitoylated, membrane-bound form known as mRPE65. mRPE65 serves as the palmitoyl donor for lecithin retinol acyl transferase (LRAT), the enzyme that catalyzes the vitamin A to all trans retinol step of the chromophore regeneration process. Both mRPE65 and sRPE65 also serve as regulatory proteins, with the ratio and concentrations of these molecules playing a role in the inhibition of 11-cis retinal synthesis. Mutations in this gene have been associated with Leber congenital amaurosis type 2 (LCA2) and retinitis pigmentosa.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :65KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :RPE65;BCO3;LCA2;RP20;mRPE65;rd12;sRPE65

Immunogen :Recombinant fusion protein of human RPE65(NP_000320.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of Mouse eye, using RPE65 antibody at 1:3460 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 30s.

Immunofluorescence analysis of Rat eye cells using RPE65 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of Mouse eye cells using RPE65 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.