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FLI1 polyclonal antibody

货号: BS7117

价格：￥ 1140

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS7117

Product Name :FLI1 polyclonal antibody

Swiss-Prot :Q01543

Host :Rabbit

Reactivity :Human, Mouse

Applications :WB, IP

Application_all :WB,1:500 - 1:2000|IP,1:50 - 1:200

Background :This gene encodes a transcription factor containing an ETS DNA-binding domain. The gene can undergo a t(11;22)(q24;q12) translocation with the Ewing sarcoma gene on chromosome 22, which results in a fusion gene that is present in the majority of Ewing sarcoma cases. An acute lymphoblastic leukemia-associated t(4;11)(q21;q23) translocation involving this gene has also been identified. Alternative splicing results in multiple transcript variants.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :51KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :FLI1;EWSR2;SIC-1;BDPLT21

Immunogen :Recombinant fusion protein of human FLI1(NP_002008.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 30s.

Immunoprecipitation analysis of 200ug extracts of Jurkat cells using 1ug FLI1 antibody . Western blot was performed from the immunoprecipitate using FLI1 antibody at a dilution of 1:1000.