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TRMT61B polyclonal antibody

货号: BS71068

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS71068

Product Name :TRMT61B polyclonal antibody

Swiss-Prot :Q9BVS5

Host :Rabbit

Reactivity :Human

Applications :WB

Application_all :WB,1:500 - 1:2000

Background :Methyltransferase that catalyzes the formation of N(1-methyladenine at position 58 (m1A58 in various tRNAs in mitochondrion, including tRNA(Leu (deciphering codons UUA or UUG, tRNA(Lys and tRNA(Ser (deciphering codons UCA, UCU, UCG or UCC. Catalyzes the formation of 1-methyladenosine at position 947 of mitochondrial 16S ribosomal RNA and this modification is most likely important for mitoribosomal structure and function. In addition to tRNA N(1-methyltransferase activity, also acts as a mRNA N(1-methyltransferase by mediating methylation of adenosine residues at the N(1 position of MT-ND5 mRNA, leading to interfere with mitochondrial translation.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :52kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :TRMT61B

Immunogen :Recombinant fusion protein of human TRMT61B(NP_060380.3).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of various lysates, using TRMT61B antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 5s.