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DNMT3A polyclonal antibody

货号: BS7101

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS7101

Product Name :DNMT3A polyclonal antibody

Swiss-Prot :Q9Y6K1

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:20

Background :CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :95kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :DNMT3A;DNMT3A2;M.HsaIIIA;TBRS;DNMT3A

Immunogen :A synthetic peptide of human DNMT3A(NP_072046.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using DNMT3A antibody .
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 90s.

Immunofluorescence analysis of A549 cells using DNMT3A antibody .

Intronic miR-140-5p contributes to beta-cypermethrin-mediated testosterone decline

JournalScience Of The Total Environment

ApplicationIF,WB,CHIP

IF7.963

SpeciesRat,Mouse

PMID34794910
DNA Hypomethylation of GR Promoters is Associated with GR Activation and BDNF/AKT/ERK1/2‐Induced Hippocampal Neurogenesis in Mice Derived From Folic‐Acid‐Supplemented Dams

JournalMolecular Nutrition & Food Research

ApplicationWB

IF4.653

SpeciesMouse

PMID30920123