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GABARAPL2 polyclonal antibody

货号: BS70915

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

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No. :BS70915

Product Name :GABARAPL2 polyclonal antibody

Swiss-Prot :P60520

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200

Background :Ubiquitin-like modifier involved in intra-Golgi traffic (By similarity. Modulates intra-Golgi transport through coupling between NSF activity and SNAREs activation (By similarity. It first stimulates the ATPase activity of NSF which in turn stimulates the association with GOSR1 (By similarity. Involved in autophagy. Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :14kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :GABARAPL2;ATG8;ATG8C;GATE-16;GATE16;GEF-2;GEF2

Immunogen :Recombinant fusion protein of human GABARAPL2(NP_009216.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of mouse brain, using GABARAPL2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 30s.

Immunohistochemistry of paraffin-embedded rat brain using GABARAPL2 antibody at dilution of 1:200 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded mouse heart using GABARAPL2 antibody at dilution of 1:200 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.