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[KO Validated] KDM5B polyclonal antibody

货号: BS70906

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS70906

Product Name :[KO Validated] KDM5B polyclonal antibody

Swiss-Prot :Q9UGL1

Host :Rabbit

Reactivity :Human, Mouse

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200

Background :This gene encodes a lysine-specific histone demethylase that belongs to the jumonji/ARID domain-containing family of histone demethylases. The encoded protein is capable of demethylating tri-, di- and monomethylated lysine 4 of histone H3. This protein plays a role in the transcriptional repression or certain tumor suppressor genes and is upregulated in certain cancer cells. This protein may also play a role in genome stability and DNA repair. Alternate splicing results in multiple transcript variants.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :176KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :KDM5B;CT31;JARID1B;PLU-1;PLU1;PPP1R98;PUT1;RBBP2H1A;RBP2-H1

Immunogen :Recombinant fusion protein of human KDM5B(NP_006609.3).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts from normal and KDM5B knockout 293T cells, using KDM5B antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 10s.

Immunofluorescence analysis of L929 using KDM5B antibody at dilution of 1:100 . Blue: DAPI for nuclear staining.