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SHH polyclonal antibody

货号: BS70878

价格：￥ 1140

货期：现货（1-2个工作日）

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详细信息检测数据实验指南文献

No. :BS70878

Product Name :SHH polyclonal antibody

Swiss-Prot :Q15465

Host :Rabbit

Reactivity :Human,Rat

Applications :WB,IHC,IF

Application_all :WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200

Background :This gene encodes a protein that is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE), a disorder in which the developing forebrain fails to correctly separate into right and left hemispheres. HPE is manifested by facial deformities. It is also thought that mutations in this gene or in its signalling pathway may be responsible for VACTERL syndrome, which is characterized by vertebral defects, anal atresia, tracheoesophageal fistula with esophageal atresia, radial and renal dysplasia, cardiac anomalies, and limb abnormalities. Additionally, mutations in a long range enhancer located approximately 1 megabase upstream of this gene disrupt limb patterning and can result in preaxial polydactyly.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :SHH polyclonal antibody detects endogenous levels of SHH protein.

BiowMW :~ 50 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Hedgehog Signaling,

Alternative Name :TPT; HHG1; HLP3; HPE3; SMMCI; TPTPS; MCOPCB5;

Immunogen :A synthetic peptide of human SHH.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of A549 cell line, using SHH antibody.

Immunohistochemistry of paraffin-embedded rat kidney using SHH antibody at dilution of 1:100 (x40 lens).

Immunohistochemistry of paraffin-embedded rat lung using SHH antibody at dilution of 1:100 (x40 lens).

Transforming growth factor‐β1 stimulates hedgehog signaling to promote epithelial–mesenchymal transition after kidney injury

JournalFEBS Journal

ApplicationIHC

IF4.739

SpeciesRat

PMID27579669