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Cyclin A2 polyclonal antibody

货号: BS70835

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS70835

Product Name :Cyclin A2 polyclonal antibody

Swiss-Prot :P20248

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200

Background :The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. This cyclin binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :55kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :CCN1;CCNA;Cyclin A2;CCNA2;cyclin-A2

Immunogen :Recombinant fusion protein of human Cyclin A2(NP_001228.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using Cyclin A2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 30s.

Immunofluorescence analysis of L929 cells using Cyclin A2 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

MicroRNA-302b-3p Suppresses Cell Proliferation Through AKT Pathway by Targeting IGF-1R in Human Gastric Cancer

JournalCellular Physiology And Biochemistry

ApplicationWB

IF0

SpeciesHuman

PMID28743112