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[KO Validated] Integrin alpha 2 (ITGA2/CD49b) polyclonal antibody

货号: BS70832

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS70832

Product Name :[KO Validated] Integrin alpha 2 (ITGA2/CD49b) polyclonal antibody

Swiss-Prot :P17301

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC, IF/ICC

Application_all :WB,1:500 - 1:2000|IHC,1:100 - 1:200|IF/ICC,1:50 - 1:200

Background :This gene encodes the alpha subunit of a transmembrane receptor for collagens and related proteins. The encoded protein forms a heterodimer with a beta subunit and mediates the adhesion of platelets and other cell types to the extracellular matrix. Loss of the encoded protein is associated with bleeding disorder platelet-type 9. Antibodies against this protein are found in several immune disorders, including neonatal alloimmune thrombocytopenia. This gene is located adjacent to a related alpha subunit gene. Alternative splicing results in multiple transcript variants.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :150KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :BR;CD49B;GPIa;HPA-5;VLA-2;VLAA2;ITGA2

Immunogen :A synthetic peptide of human alpha 2 (ITGA2/CD49b)(NP_002194.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using Integrin alpha 2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 90s.

Western blot analysis of extracts from normal and Integrin alpha 2 knockout 293T cells, using Integrin alpha 2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 90s.

Immunohistochemistry of paraffin-embedded human lung cancer using [KO Validated] Integrin alpha 2 Rabbit pAb at dilution of 1:50 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Quantitative proteomics reveals potential anti-inflammatory protein targets of radial extracorporeal shock wave therapy in TNF-α-induced model of acute inflammation in primary human tenocytes

JournalHeliyon

ApplicationWB

IF3.776

SpeciesHuman

PMID36506366