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Argonaute-2 polyclonal antibody

货号: BS70734

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS70734

Product Name :Argonaute-2 polyclonal antibody

Swiss-Prot :Q9UKV8

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC

Application_all :WB,1:500 - 1:1000|IHC,1:50 - 1:200

Background :This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing. Multiple transcript variants encoding different isoforms have been found for this gene.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :97kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :AGO2;CASC7;EIF2C2;LINC00980;PPD;Q10;protein argonaute-2;Argonaute 2;EIF2C2

Immunogen :A synthetic peptide of human Argonaute-2(NP_036286.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using Argonaute-2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 30s.

Immunohistochemistry of paraffin-embedded human stomach using Argonaute-2 antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded mouse liver using Argonaute-2 antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Microvesicle‐Shuttled miR‐130b Reduces Fat Deposition in Recipient Primary Cultured Porcine Adipocytes by Inhibiting PPAR‐γ Expression

JournalJournal Of Cellular Physiology

ApplicationWB

IF5.546

SpeciesPig

PMID24311275
The Long Noncoding RNA MALAT1 Induces Tolerogenic Dendritic Cells and Regulatory T Cells via miR155/Dendritic Cell-Specific Intercellular Adhesion Molecule-3 Grabbing Nonintegrin/IL10 Axis

JournalFrontiers in Immunology

ApplicationIP

IF5.085

SpeciesMouse

PMID30150986