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TNRC6A polyclonal antibody

货号: BS70720

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS70720

Product Name :TNRC6A polyclonal antibody

Swiss-Prot :Q8NDV7

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:1000|IF/ICC,1:50 - 1:100

Background :This gene encodes a member of the trinucleotide repeat containing 6 protein family. The protein functions in post-transcriptional gene silencing through the RNA interference (RNAi) and microRNA pathways. The protein associates with messenger RNAs and Argonaute proteins in cytoplasmic bodies known as GW-bodies or P-bodies. Inhibiting expression of this gene delocalizes other GW-body proteins and impairs RNAi and microRNA-induced gene silencing.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :180kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :TNRC6A;CAGH26;GW1;GW182;TNRC6

Immunogen :A synthetic peptide of human TNRC6A(NP_055309.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using TNRC6A antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 90s.

Immunofluorescence analysis of U2OS cells using TNRC6A antibody at dilution of 1:100. Blue: DAPI for nuclear staining.