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ELAVL3 polyclonal antibody

货号: BS70716

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS70716

Product Name :ELAVL3 polyclonal antibody

Swiss-Prot :Q14576

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC, IF/ICC

Application_all :WB,1:500 - 1:1000|IHC,1:50 - 1:200|IF/ICC,1:50 - 1:200

Background :A member of the ELAVL protein family, ELAV-like 3 is a neural-specific RNA-binding protein which contains three RNP-type RNA recognition motifs. The observation that ELAVL3 is one of several Hu antigens (neuronal-specific RNA-binding proteins) recognized by the anti-Hu serum antibody present in sera from patients with paraneoplastic encephalomyelitis and sensory neuronopathy (PEM/PSN) suggests it has a role in neurogenesis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :37KDaa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :ELAVL3;HUC;HUCL;PLE21

Immunogen :A synthetic peptide of human ELAVL3(NP_001411.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of various lysates, using ELAVL3 antibody at 1:550 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 0.8s.

Immunohistochemistry of paraffin-embedded rat brain using ELAVL3 antibody at dilution of 1:200 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded mouse brain using ELAVL3 antibody at dilution of 1:200 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunofluorescence analysis of SH-SY5Y cells using ELAVL3 Rabbit pAb at dilution of 1:100 . Blue: DAPI for nuclear staining.