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HNRNPA0 polyclonal antibody

货号: BS70709

价格：￥ 1140

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

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No. :BS70709

Product Name :HNRNPA0 polyclonal antibody

Swiss-Prot :Q13151

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IP

Application_all :WB,1:500 - 1:1000|IP,1:50 - 1:100

Background :This gene belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind RNAs, followed by a glycine-rich C-terminus.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :37kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :HNRNPA0;HNRPA0

Immunogen :Recombinant fusion protein of human HNRNPA0(NP_006796.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using HNRNPA0 antibody at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 1s.

Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug HNRNPA0 antibody . Western blot was performed from the immunoprecipitate using HNRNPA0 antibody at a dilution of 1:1000.