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[KO Validated] PPP1CC polyclonal antibody

货号: BS70591

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS70591

Product Name :[KO Validated] PPP1CC polyclonal antibody

Swiss-Prot :P36873

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IF/ICC

Application_all :WB,1:200 - 1:2000|IF/ICC,1:50 - 1:200

Background :The protein encoded by this gene belongs to the protein phosphatase family, PP1 subfamily. PP1 is an ubiquitous serine/threonine phosphatase that regulates many cellular processes, including cell division. It is expressed in mammalian cells as three closely related isoforms, alpha, beta/delta and gamma, which have distinct localization patterns. This gene encodes the gamma isozyme. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :38kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :PPP1CC;PP-1G;PP1C;PPP1G

Immunogen :Recombinant fusion protein of human PPP1CC(NP_002701.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using PPP1CC antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 90s.

Western blot analysis of extracts from normal and PPP1CC knockout HeLa cells, using PPP1CC antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 1s.

Immunofluorescence analysis of H9C2 cells using [KO Validated] PPP1CC Rabbit pAb at dilution of 1:100 . Blue: DAPI for nuclear staining.

Immunofluorescence analysis of L929 cells using [KO Validated] PPP1CC Rabbit pAb at dilution of 1:100 . Blue: DAPI for nuclear staining.