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[KO Validated] PPP1CC polyclonal antibody

货号: BS70591
价格 1140
货期 现货(1-2个工作日)
25ul 50ul 100ul 200ul
No. :BS70591
Product Name :[KO Validated] PPP1CC polyclonal antibody
Swiss-Prot :P36873
Host :Rabbit
Reactivity :Human, Mouse, Rat
Applications :WB, IF/ICC
Application_all :WB,1:200 - 1:2000|IF/ICC,1:50 - 1:200
Background :The protein encoded by this gene belongs to the protein phosphatase family, PP1 subfamily. PP1 is an ubiquitous serine/threonine phosphatase that regulates many cellular processes, including cell division. It is expressed in mammalian cells as three closely related isoforms, alpha, beta/delta and gamma, which have distinct localization patterns. This gene encodes the gamma isozyme. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :Polyclonal Antibodies
BiowMW :38kDa
Note :For research use only, not for use in diagnostic procedure.
Alternative Name :PPP1CC;PP-1G;PP1C;PPP1G
Immunogen :Recombinant fusion protein of human PPP1CC(NP_002701.1).
Conjugate :Unconjugated
Modification :Unmodification
Western blot analysis of extracts of various cell lines, using PPP1CC antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 90s.
Western blot analysis of extracts from normal and PPP1CC knockout HeLa cells, using PPP1CC antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 1s.
Immunofluorescence analysis of H9C2 cells using [KO Validated] PPP1CC Rabbit pAb at dilution of 1:100 . Blue: DAPI for nuclear staining.
Immunofluorescence analysis of L929 cells using [KO Validated] PPP1CC Rabbit pAb at dilution of 1:100 . Blue: DAPI for nuclear staining.

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400-660-9091

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com