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ENTPD1/CD39 polyclonal antibody

货号: BS70562

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

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No. :BS70562

Product Name :ENTPD1/CD39 polyclonal antibody

Swiss-Prot :P49961

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC, IF/ICC

Application_all :WB,1:200 - 1:1000|IHC,1:50 - 1:200|IF/ICC,1:50 - 1:200

Background :The protein encoded by this gene is a plasma membrane protein that hydrolyzes extracellular ATP and ADP to AMP. Inhibition of this protein's activity may confer anticancer benefits. Several transcript variants encoding different isoforms have been found for this gene.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :80KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :ENTPD1;ATPDase;CD39;NTPDase-1;SPG64

Immunogen :Recombinant fusion protein of human ENTPD1/CD39(NP_001157655.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using ENTPD1/CD39 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 180s.

Immunohistochemistry of paraffin-embedded human liver using ENTPD1/CD39 Rabbit pAb at dilution of 1:25 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded human placenta using ENTPD1/CD39 Rabbit pAb at dilution of 1:25 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunofluorescence analysis of Raw264.7 cells using ENTPD1/CD39 Polyclonal Antibody at dilution of 1:100 . Blue: DAPI for nuclear staining.