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ATP6V1B2 polyclonal antibody

货号: BS70561

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS70561

Product Name :ATP6V1B2 polyclonal antibody

Swiss-Prot :P21281

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB

Application_all :WB,1:500 - 1:2000

Background :This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. The protein encoded by this gene is one of two V1 domain B subunit isoforms and is the only B isoform highly expressed in osteoclasts.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :57kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :ATP6V1B2;ATP6B1B2;ATP6B2;DOOD;HO57;VATB;VPP3;Vma2;ZLS2

Immunogen :Recombinant fusion protein of human ATP6V1B2(NP_001684.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using ATP6V1B2 Antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 30s.