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LRPPRC polyclonal antibody

货号: BS70542

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS70542

Product Name :LRPPRC polyclonal antibody

Swiss-Prot :P42704

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC, IF/ICC

Application_all :WB,1:1000 - 1:2000|IHC,1:100 - 1:200|IF/ICC,1:100 - 1:200

Background :This gene encodes a leucine-rich protein that has multiple pentatricopeptide repeats (PPR). The precise role of this protein is unknown but studies suggest it may play a role in cytoskeletal organization, vesicular transport, or in transcriptional regulation of both nuclear and mitochondrial genes. The protein localizes primarily to mitochondria and is predicted to have an N-terminal mitochondrial targeting sequence. Mutations in this gene are associated with the French-Canadian type of Leigh syndrome.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :130kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :LRPPRC;CLONE-23970;GP130;LRP130;LSFC

Immunogen :Recombinant fusion protein of human LRPPRC(NP_573566.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using LRPPRC antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 5s.

Immunohistochemistry of paraffin-embedded human breast cancer using LRPPRC Rabbit pAb at dilution of 1:250 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded human colon carcinoma using LRPPRC Rabbit pAb at dilution of 1:250 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Confocal immunofluorescence analysis of Hela cells using LRPPRC Polyclonal Antibody at dilution of 1:400. Blue: DAPI for nuclear staining.

XPO1 inhibitor KPT-330 synergizes with Bcl-xL inhibitor to induce cancer cell apoptosis by perturbing rRNA processing and Mcl-1 protein synthesis

JournalCell Death & Disease

ApplicationWB

IF5.959

SpeciesMouse,Human

PMID31113936