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Cleaved-MMP-14 (Y112) polyclonal antibody（KU）

货号: BS7040

价格：￥ 1140

货期：现货（1-2个工作日）

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详细信息检测数据实验指南文献

No. :BS7040

Product Name :Cleaved-MMP-14 (Y112) polyclonal antibody（KU）

Swiss-Prot :P50281

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB

Application_all :WB: 1:500~1:1000

Background :The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, fibronectin, laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. Membrane-type matrix metalloproteinases, including MT-MMP-1 (also designated MMP-14), MT-MMP-2 (also designated MMP-15), MT-MMP-3 (also designated MMP-16) and MT-MMP-4 (also designated MMP-17) are type I membrane proteins that function to activate other MMPs. MT-MMP activation appears to be mediated by members of the proprotein convertase family, suggesting that a proprotein convertase/MT-MMP/MMP cascade may be involved in the regulation of ECM turnover.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Cleaved-MMP-14 (Y112) polyclonal antibody detects endogenous levels of Pro-MMP-14 (65 kDa) or Cleaved-MMP-14 (53 kDa) protein.

BiowMW :~ 53, 65 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Contribution of ECM and Cytoskeletal Factors to EMT,

Alternative Name :Matrix metalloproteinase-14; MMP 14; MMP-X1; Membrane-type matrix metalloproteinase 1; MT-MMP 1; MTMMP1; Membrane-type-1 matrix metalloproteinase; MT1-MMP; MT1MMP; MMP14

Immunogen :Synthetic peptide, corresponding to amino acids 100-150 of Human MMP-14.

Conjugate :Unconjugated

Modification :Cleaved

Western blot (WB) analysis of Cleaved-MMP-14 (Y112) pAb at 1:500 dilution Lane1:HCT116 whole cell lysate(40ug) Lane2:MCF-7 whole cell lysate(40ug) Lane3:The Uterus tissue lysate of Rat(40ug) Lane4:The Testis tissue lysate of Mouse(40ug)

miR‑133a suppresses cell proliferation, migration and invasion in human lung cancer by targeting MMP‑14

JournalOncology Reports

ApplicationWB

IF3.906

SpeciesHuamn

PMID23783274
Hypoxic tubular epithelial cells regulate the angiogenesis of HMEC-1 cells via mediation of Rab7/MMP-2 axis

JournalAging-US

ApplicationWB

IF5.682

SpeciesMouse,Human

PMID34695807