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LRP5 polyclonal antibody

货号: BS70101

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS70101

Product Name :LRP5 polyclonal antibody

Swiss-Prot :O75197

Host :Rabbit

Reactivity :Mouse,Rat

Applications :WB IF/ICC

Application_all :WB: 1:100~1:500 IF/ICC: 1:50~1:200

Background :LRP5 and LRP6 are single-pass transmembrane proteins belonging to the low-density lipoprotein receptor (LDLR)-related protein family. Unlike other members of the LDLR family, LRP5 and LRP6 have four EGF and three LDLR repeats in the extracellular domain, and proline-rich motifs in the cytoplasmic domain (1). They function as co-receptors for Wnt and are required for the canonical Wnt/β-catenin signaling pathway (2,3). LRP5 and LRP6 are highly homologous and have redundant roles during development (4,5). The activity of LRP5 and LRP6 can be inhibited by the binding of some members of the Dickkopf (DKK) family of proteins (6,7). Upon stimulation with Wnt, LRP6 is phosphorylated at multiple sites including Thr1479, Ser1490, and Thr1493 by kinases such as GSK-3 and CK1 (8-10). Phosphorylated LRP6 recruits axin to the membrane and presumably activates β-catenin signaling (8-10).LRP5 is involved in the regulation of bone homeostasis. Mutations and polymorphisms in LPR5 are associated with bone diseases like osteoporosis-pseudoglioma syndrome and high-bone-mass disorders (11-13). In addition, mutations in LRP5 are found in patients with hyperparathyroid tumor and breast cancer (14,15).

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :LRP5 polyclonal antibody detects endogenous levels of LRP5 protein.

BiowMW :~ 180-200 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Wntβ catenin signaling，Regulation of Microtubule Dynamics,

Alternative Name :LRP5; BMND1; EVR1; EVR4; HBM; LR3; LRP7; OPPG; OPS; OPTA1; VBCH2

Immunogen :A synthetic peptide corresponding to a sequence within amino acids 1400-1500 of human LRP5 (NP_002326.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using LRP5 antibody at 1:500 dilution.

Immunofluorescence analysis of NIH/3T3 cells using LRP5 pAb at dilution of 1:200 (40x lens).

Immunofluorescence analysis of PC12 cells using LRP5 pAb at dilution of 1:200 (40x lens).