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CRHBP polyclonal antibody

货号: BS6948

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS6948

Product Name :CRHBP polyclonal antibody

Swiss-Prot :P24387

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC-P IF/ICC

Application_all :WB: 1:1000-1:5000 IHC-P: 1:100-1:500 IF/ICC: 1:50-1:100

Background :Response to stress in mammals requires an intact hypothalamic-pituitary-adrenal axis. The proximal part of the response is mediated by secretion of corticotropin-releasing hormone (CRH) by the paraventricular nucleus of the hypothalamus. CRH is a 41 amino acid peptide derived by enzymatic cleavage from a 191 amino acid preprohormone. CRH is produced not only in the hypothalamus but also in peripheral tissues, such as T lymphocytes; it is highly expressed in human placenta. Glucocorticoids stimulate placental CRH synthesis and secretion in primary cultures of human placenta. This stimulation is in contrast to the glucocorticoid suppression of CRH expression in hypothalamus. The gene which encodes CRH maps to human chromosome 8q13. Human plasma contains a CRH-binding protein, CRH-BP (also designated CRF-BP) which inactivates CRH and which may prevent inappropriate pituitary-adrenal stimulation in pregnancy. The gene which encodes CRF-BP maps to human chromosome 5q11.2-q13.3 .

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :CRHBP polyclonal antibody detects endogenous levels of CRHBP protein.

BiowMW :~ 37 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :CRFBP; CRF-BP

Immunogen :Recombinant fusion protein containing a sequence corresponding to amino acids 25-322 of human CRHBP (NP_001873.2).

Conjugate :Unconjugated

Modification :Unmodified

Western blot analysis of Rat brain, using CRHBP pAb at 1:2000 dilution.

Immunohistochemistry analysis of CRHBP in paraffin-embedded mouse kidney tissue using CRHBP pAb at a dilution of 1:300 (40x lens).

Immunohistochemistry analysis of CRHBP in paraffin-embedded rat kidney tissue using CRHBP pAb at a dilution of 1:300 (40x lens).

Immunofluorescence analysis of HeLa cells using CRHBP antibody. Blue: DAPI for nuclear staining.