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[KO Validated] CIRBP polyclonal antibody

货号: BS6943

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS6943

Product Name :[KO Validated] CIRBP polyclonal antibody

Swiss-Prot :Q14011

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC, IF/ICC

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:100|IF/ICC,1:10 - 1:100

Background :Cold-inducible mRNA binding protein that plays a protective role in the genotoxic stress response by stabilizing transcripts of genes involved in cell survival. Acts as a translational activator. Seems to play an essential role in cold-induced suppression of cell proliferation. Binds specifically to the 3'-untranslated regions (3'-UTRs of stress-responsive transcripts RPA2 and TXN. Acts as a translational repressor (By similarity. Promotes assembly of stress granules (SGs, when overexpressed.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :18kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :CIRBP;CIRP

Immunogen :Recombinant fusion protein of human CIRBP(NP_001271.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using CIRBP antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 90s.

Western blot analysis of extracts from normal and CIRBP knockout 293T cells, using CIRBP antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 180s.

Immunohistochemistry of paraffin-embedded rat testis using CIRBP antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded human breast cancer using CIRBP antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Molecular characterization of CIRBP from Takifugu fasciatus and its potential roles in cold-induced liver damage

Journal10.1016/j.ijbiomac.2024.136492

ApplicationWB,IF

IF7.7

SpeciesFish

PMID39393746