Product Name :Histone H4 (AcK16) polyclonal antibody
Reactivity :Human,Mouse,Rat
Application_all :WB:1:500~1:1000 IHC:1:50~1:200 IF:1:50~1:200
Background :Histone proteins H3, H4, H2A, and H2B function as building blocks to package eukaryotic DNA into repeating nucleosome units that are folded in higher order chromatin fibers. The nucleosome is composed of an octamer containing a H3/H4 tetramer and two H2A/H2B dimers, surrounded by approximately 146 base pairs of DNA. A diverse and elaborate array of post-translational modifications including acetylation, phosphorylation, methylation, ubiquitination, and ADP-ribosylation occurs on the N-terminal tail domains of histones. Methylation of position-specific lysine residues in histone N termini is a central modification for regulating epigenetic transitions in chromatin. Each methylatable lysine residue can exist in a mono, di, or tri methylated state. Arginine resdiues can also by mono or di methylated.
Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :Histone H4 (AcK16) polyclonal antibody detects endogenous levels of Histone H4 protein.
Note :For research use only, not for use in diagnostic procedure.
Pathway :Examples of Crosstalk Between Post-translational Modifications,
Alternative Name :Histone H4; HIST1H4A; H4/A, H4FA; HIST1H4B; H4/I; H4FI; HIST1H4C; H4/G, H4FG; HIST1H4D; H4/B, H4FB; HIST1H4E; H4/J, H4FJ; HIST1H4F; H4/C; H4FC; HIST1H4H; H4/H; H4FH; HIST1H4I; H4/M; H4FM; HIST1H4J; H4/E, H4FE; HIST1H4K; H4/D, H4FD; HIST1H4L; H4/K; H4FK; HIST2H4A; H4/N; H4F2; H4FN; HIST2H4; HIST2H4B; H4/O; H4FO; HIST4H4
Immunogen :Synthetic peptide, corresponding to Human Histone H4.
Modification :Unmodification
