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MDM2 polyclonal antibody

货号: BS6818

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS6818

Product Name :MDM2 polyclonal antibody

Swiss-Prot :Q00987

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF

Application_all :WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200

Background :p53 is the most commonly mutated gene in human cancer identified to date. Expression of p53 leads to inhibition of cell growth by preventing progression of cells from G1 to S phase of the cell cycle. Most importantly, p53 functions to cause arrest of cells in the G1 phase of the cell cycle following any exposure of cells to DNA-damaging agents. The MDM2 (murine double minute-2) protein was initially identified as an oncogene in a murine transformation system. MDM2 functions to bind p53 and block p53-mediated transactivation of cotransfected reporter constructs. The MDM2 gene is amplified in a high percentage of human sarcomas that retain wildtype p53 and tumor cells that overexpress MDM2 can tolerate high levels of p53 expression. These findings argue that MDM2 overexpression represents at least one mechanism by which p53 function can be abrogated during tumorigenesis.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :MDM2 polyclonal antibody detects endogenous levels of MDM2 protein.

BiowMW :~ 50 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Examples of Crosstalk Between Post-translational Modifications,Cell Cycle G2 M DNA Damage Signaling Pathway,

Alternative Name :E3 ubiquitin-protein ligase Mdm2; Double minute 2 protein; Hdm2; Oncoprotein Mdm2; p53-binding protein Mdm2; MDM2

Immunogen :Recombinant full length Human MDM2.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using MDM2 antibody.

Immunohistochemistry (IHC) analysis of MDM2 polyclonal antibody in paraffin-embedded Human Stomach tissue.

D-peptide inhibitors of the p53–MDM2 interaction for targeted molecular therapy of malignant neoplasms

JournalProceedings Of The National Academy Of Sciences Of The United States Of Ame

ApplicationWB

IF9.58

SpeciesHuman

PMID20660730
Phosphorylated Hsp27 activates ATM-dependent p53 signaling and mediates the resistance of MCF-7 cells to doxorubicin-induced apoptosis

JournalCellular Signalling

ApplicationWB

IF3.388

SpeciesHuman

PMID23357534
Protosappanin B protects PC12 cells against oxygen–glucose deprivation-induced neuronal death by maintaining mitochondrial homeostasis via induction of ubiquitin-dependent p53 protein degradation

JournalEuropean Journal Of Pharmacology

ApplicationIP

IF3.17

SpeciesRat

PMID25657114
Curcumin induces p53-independent necrosis in H1299 cells via a mitochondria-associated pathway

JournalMolecular Medicine Reports

ApplicationWB

IF2.1

SpeciesHuman

PMID26460892