Product Name :NLRP1 polyclonal antibody
Reactivity :Human, Mouse, Rat
Applications :WB, IHC, IF/ICC
Application_all :WB (1/500 - 1/1000), IHC (1/50 - 1/200), IF/ICC (1/50 - 1/200)
Background :NALP1 (DEFCAP/NAC/CARD7) is an NLR (Nod-like receptor) family member that has been implicated in the regulation of apoptosis and inflammatory responses. Structurally, NALP contains an amino-terminal PYRIN domain, followed by a nucleotide-binding site (NBS), a leucine-rich repeat region (LRR), and a carboxy-terminal CARD domain. NALP1 interacts strongly with caspase-2 and weakly with caspase-9, and induces apoptosis when overexpressed. Similar to a related Ced-4 family member Apaf-1, it was also shown to be involved in cytochrome c-dependent caspase activation. It has also been shown to be part of the "inflammasome" comprised of caspase-1, caspase-5, and Pycard/ASC, which is critical in the processing of pro-inflammatory cytokines like IL-1β. Two major isoforms were identified for NALP1, which differ in a 44 amino acid region within the LRR. In addition, like NALP3, a short NALP1 isoform lacking the LRR (NALP1s) likely exists. Polymorphisms in NALP1 have been associated with autoimmune diseases and susceptibility to toxins.
NALP1 is autoproteolytically processed into a large N-terminal and a small C-terminal fragment, which are non-covalently associated. Upon activation, the C-terminal is released and forms inflammasomes with other proteins.
Product :Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Purification&Purity :The antibody was purified by immunogen affinity chromatography.
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :Recognizes endogenous levels of NLRP1 protein.
Note :For research use only, not for use in diagnostic procedure.
Alternative Name :CARD7; DEFCAP; KIAA0926; NAC; NALP1; NACHT LRR and PYD domains-containing protein 1; Caspase recruitment domain-containing protein 7; Death effector filament-forming ced-4-like apoptosis protein; Nucleotide-binding domain and caspase recruitment domain
Immunogen :Recombinant protein corresponding to human NLRP1. The exact sequence is proprietary.
Modification :Unmodification
