产品类别

联系我们

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

FOXO4 (Phospho-S262) polyclonal antibody

货号: BS67590

价格：￥ 1350

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS67590

Product Name :FOXO4 (Phospho-S262) polyclonal antibody

Swiss-Prot :P98177

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC

Application_all :WB (1/500 - 1/1000), IHC (1/50 - 1/200)

Background :The Forkhead family of transcription factors is involved in tumorigenesis of rhabdomyosarcoma and acute leukemias . Within the family, three members (FoxO1, FoxO4, and FoxO3a) have sequence similarity to the nematode orthologue DAF-16, which mediates signaling via a pathway involving IGFR1, PI3K, and Akt . Active forkhead members act as tumor suppressors by promoting cell cycle arrest and apoptosis. Increased expression of any FoxO member results in the activation of the cell cycle inhibitor p27 Kip1. Forkhead transcription factors also play a part in TGF--mediated upregulation of p21 Cip1, a process negatively regulated through PI3K . Increased proliferation results when forkhead transcription factors are inactivated through phosphorylation by Akt at Thr24, Ser256, and Ser319, which results in nuclear export and inhibition of transcription factor activity . Forkhead transcription factors can also be inhibited by the deacetylase sirtuin (SirT1).

Product :Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Purification&Purity :The antibody was purified by immunogen affinity chromatography.

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Recognizes endogenous levels of FOXO4 with a phosphorylation site at S262 protein.

BiowMW :~ 62 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Insulin &Glucose Signaling

Alternative Name :AFX; AFX1; MLLT7; Forkhead box protein O4; Fork head domain transcription factor AFX1

Immunogen :KLH-conjugated synthetic peptide encompassing a sequence within the center region of human FOXO4 with a phosphorylation site at S262. The exact sequence is proprietary.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot analysis of FOXO4 (pS262) expression in mouse muscle (A), rat brain (B), rat muscle (C) whole cell lysates.

Immunohistochemical analysis of FOXO4 (pS262) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.