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p27 Kip1 polyclonal antibody

货号: BS67448

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS67448

Product Name :p27 Kip1 polyclonal antibody

Swiss-Prot :P46527

Host :Rabbit

Reactivity :Human, Rat, Dog

Applications :WB, IHC

Application_all :WB (1/500 - 1/1000), IHC (1/50 - 1/200)

Background :p27 Kip1 is a member of the Cip/Kip family of cyclin-dependent kinase inhibitors. Like its relatives, p57 Kip2 and p21 Waf1/Cip1, the ability to enforce the G1 restriction point is derived from its inhibitory binding to CDK2/cyclin E and other CDK/cyclin complexes. Expression levels of p27 are upregulated in quiescent cells and in cells treated with cAMP or other negative cell cycle regulators. Downregulation of p27 can be induced by treatment with interleukin-2 or other mitogens; this involves phosphorylation of p27 and its degradation by the ubiquitin-proteasome pathway.

Product :Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Purification&Purity :The antibody was purified by immunogen affinity chromatography.

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Recognizes endogenous levels of p27 Kip1 protein.

BiowMW :~ 27 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :ErbB HER Signaling,Cell Cycle Control G1 S Checkpoin,MAPK Erk in Growth and Differentiation Pathway(,PI3K AKT signaling.

Alternative Name :KIP1; Cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor p27; p27Kip1

Immunogen :KLH-conjugated synthetic peptide encompassing a sequence within the center region of human p27 Kip1. The exact sequence is proprietary.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of p27 Kip1 expression in Hela (A), HEK293T (B), C6 (C) whole cell lysates.

Immunohistochemical analysis of p27 Kip1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.