Product Name :14-3-3 sigma (Phospho-S186) polyclonal antibody
Reactivity :Human, Mouse, Rat, Bovine, Pig, Sheep
Application_all :WB (1/500 - 1/1000)
Background :The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways . 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, β, γ, ε, σ, ζ, τ, and η that have been identified in mammals. The initially described α and δ isoforms are confirmed to be phosphorylated forms of β and ζ, respectively . Through their amino-terminal α helical region, 14-3-3 proteins form homo- or heterodimers that interact with a wide variety of proteins: transcription factors, metabolic enzymes, cytoskeletal proteins, kinases, phosphatases, and other signaling molecules . The interaction of 14-3-3 proteins with their targets is primarily through a phospho-Ser/Thr motif. However, binding to divergent phospho-Ser/Thr motifs, as well as phosphorylation independent interactions has been observed. 14-3-3 binding masks specific sequences of the target protein, and therefore, modulates target protein localization, phosphorylation state, stability, and molecular interactions . 14-3-3 proteins may also induce target protein conformational changes that modify target protein function . Distinct temporal and spatial expression patterns of 14-3-3 isoforms have been observed in development and in acute response to extracellular signals and drugs, suggesting that 14-3-3 isoforms may perform different functions despite their sequence similarities . Several studies suggest that 14-3-3 isoforms are differentially regulated in cancer and neurological syndromes.
Product :Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Purification&Purity :The antibody was purified by immunogen affinity chromatography.
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :Recognizes endogenous levels of 14-3-3 sigma with a site at pS186 protein.
Note :For research use only, not for use in diagnostic procedure.
Pathway :Regulation of Actin Dynamics,Regulation of Apoptosis,Cell Cycle G2 M DNA Damage Signaling Pathway,MAPK Erk in Growth and Differentiation Pathway(,hippo Signaling,Mitochondrial Control of Apoptosis,PI3K AKT signaling,SAPK JNK Signaling Cascades.
Alternative Name :HME1; 14-3-3 protein sigma; Epithelial cell marker protein 1; Stratifin
Immunogen :KLH-conjugated synthetic peptide encompassing a sequence within the center region of human 14-3-3 sigma with a site at pS186. The exact sequence is proprietary.
Modification :Phosphorylation
