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DAP Kinase 1 (Phospho-S308) polyclonal antibody

货号: BS67434

价格：￥ 1350

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

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No. :BS67434

Product Name :DAP Kinase 1 (Phospho-S308) polyclonal antibody

Swiss-Prot :P53355

Host :Rabbit

Reactivity :Human, Mouse, Rat, Pig

Applications :WB

Application_all :WB (1/500 - 1/1000)

Background :Death-associated protein kinase (DAPK1) is a Ca2+/calmodulin-regulated serine/threonine kinase that participates in a wide range of apoptotic signals including interferon-γ, tumor necrosis factor α, Fas, activated c-Myc, and detachment from the extracellular matrix. In addition to the kinase domain and calmodulin regulatory segment, DAPK1 also has eight ankyrin repeats, a cytoskeleton binding region, and a conserved death domain . Deletion of the calmodulin-regulatory domain generates a constitutively active mutant kinase. Ectopic expression of wild-type DAPK1 induced cell death in HeLa cells. Conversely, expression of a catalytically inactive mutant protected cells from interferon-γ-induced cell death . The catalytic domain of DAPK1 has very high sequence similarity to vertebrate myosin light chain kinase (MLCK) and a RXX(S/T)X motif derived from myosin light chain protein was shown to be phosphorylated in vitro by DAPK1 .Epigenetic silencing of DAPK1 by promoter methylation has been observed in cases of chronic lymphocytic leukemia.

Product :Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Purification&Purity :The antibody was purified by immunogen affinity chromatography.

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Recognizes endogenous levels of DAP Kinase 1 with a site at pS308 protein.

BiowMW :~ 160 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Death Receptor Signaling,MAPK Erk in Growth and Differentiation Pathway(.

Alternative Name :DAPK; Death-associated protein kinase 1; DAP kinase 1

Immunogen :KLH-conjugated synthetic peptide encompassing a sequence within the center region of human DAP Kinase 1 with a site at pS308. The exact sequence is proprietary.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot analysis of DAP Kinase 1 (pS308) expression in H1792 (A), A549 (B) whole cell lysates.