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Topoisomerase 2 alpha (Phospho-S1213) polyclonal antibody

货号: BS67431

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :BS67431

Product Name :Topoisomerase 2 alpha (Phospho-S1213) polyclonal antibody

Swiss-Prot :P11388

Host :Rabbit

Reactivity :Human

Applications :WB

Application_all :WB (1/500 - 1/1000)

Background :DNA topoisomerases I and II are nuclear enzymes; type II consists of two highly homologous isoforms: topoisomerase IIα and IIβ. These enzymes regulate the topology of DNA, maintain genomic integrity, and are essential for processes such as DNA replication, recombination, transcription, and chromosome segregation by allowing DNA strands to pass through each other. Topoisomerase I nicks and rejoins one strand of the duplex DNA, while topoisomerase II transiently breaks and closes double-stranded DNA . Topoisomerases are very susceptible to various stresses. Acidic pH or oxidative stress can convert topoisomerases to DNA-breaking nucleases, causing genomic instability and cell death. DNA-damaging topoisomerase targeting drugs (e.g., etoposide) also convert topoisomerases to nucleases, with the enzyme usually trapped as an intermediate that is covalently bound to the 5+ end of the cleaved DNA strand(s). Research studies have shown that this intermediate leads to genomic instability and cell death. Thus, agents that target topoisomerases are highly sought after cancer chemotherapeutic drugs. Ca2+-regulated phosphorylation of topoisomerase IIα at Ser1106 modulates the activity of this enzyme and its sensitivity to targeting drugs.

Product :Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Purification&Purity :The antibody was purified by immunogen affinity chromatography.

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Recognizes endogenous levels of Topoisomerase 2 alpha with a site at pS1213 protein.

BiowMW :~ 190 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Cell Cycle G2 M DNA Damage Signaling Pathway.

Alternative Name :TOP2; DNA topoisomerase 2-alpha; DNA topoisomerase II alpha isozyme

Immunogen :KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Topoisomerase 2 alpha with a site at pS1213. The exact sequence is proprietary.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot analysis of Topoisomerase 2 alpha (pS1213) expression in SHSY5Y (A), Myla2059 (B) whole cell lysates.