Product Name :CD4 (Phospho-S433) polyclonal antibody
Reactivity :Human, Mouse, Rat, Dog, Monkey
Application_all :WB (1/500 - 1/1000), IF/ICC (1/50 - 1/200)
Background :Cluster of Differentiation 4 (CD4) is a glycoprotein composed of an amino-terminal extracellular domain (four domains: D1-D4 with Ig-like structures), a transmembrane part, and a short cytoplasmic tail. CD4 is expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells, and plays an important role in the development and activation of T cells. On T cells, CD4 is the co-receptor for the T cell receptor (TCR), and these two distinct structures recognize the Antigen–Major Histocompatibility Complex (MHC). Specifically, the D1 domain of CD4 interacts with the β2-domain of the MHC class II molecule. CD4 ensures specificity of the TCR–antigen interaction, prolongs the contact between the T cell and the antigen presenting cell, and recruits the tyrosine kinase Lck, which is essential for T cell activation.
Product :Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Purification&Purity :The antibody was purified by immunogen affinity chromatography.
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :Recognizes endogenous levels of CD4 with a site at pS433 protein.
Note :For research use only, not for use in diagnostic procedure.
Pathway :T cell receptor signaling.
Alternative Name :T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3; CD4
Immunogen :KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human CD4 with a site at pS433. The exact sequence is proprietary.
Modification :Phosphorylation
