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SPARC polyclonal antibody

货号: BS6738

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS6738

Product Name :SPARC polyclonal antibody

Swiss-Prot :P09486

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC-P IF/ICC

Application_all :WB 1:500 - 1:2000 IHC-P 1:50 - 1:100 IF /ICC 1:50 - 1:100

Background :SPARC (for secreted protein acidic and rich in cysteine) is a phosphorylated, acidic, glycine-rich glycoprotein that is secreted by endothelial cells and is present in large amounts in the parietal endoderm of mouse embryos and in human placenta. It is identical to osteonectin, a protein important to bone calcification that is highly conserved between species. SPARC, which can be selectively expressed by the endothelium in response to certain types of injury, induces rounding in adherent endothelial cells in vitro. It regulates endothelial barrier function through F-Actin-dependent changes in cell shape, coincident with the appearance of intercellular gaps, which provide a paracellular pathway for extravasation of macromolecules.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :SPARC polyclonal antibody detects endogenous levels of SPARC protein.

BiowMW :~ 35 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Contribution of ECM and Cytoskeletal Factors to EMT,

Alternative Name :SPARC; Basement-membrane protein 40; BM-40; Osteonectin; Short name=ON; Secreted protein acidic and rich in cysteine; SPARC; ON

Immunogen :Recombinant fusion protein containing a sequence corresponding to amino acids 18-303 of human SPARC (NP_003109.1).

Conjugate :Unconjugated

Modification :Unmodified

Western blot analysis of extracts of A375 cells, using SPARC antibody at 1:3000 dilution.

Immunofluorescence analysis of C6 cells using SPARC Polyclonal Antibody at dilution of 1:100 (40x lens).

Immunofluorescence analysis of L-929 cells using SPARC Polyclonal Antibody at dilution of 1:100 (40x lens).