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ISCU polyclonal antibody

货号: BS67352

价格：￥ 1140

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS67352

Product Name :ISCU polyclonal antibody

Swiss-Prot :Q9H1K1

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB

Application_all :WB (1/500 - 1/2000)

Background :Scaffold protein for the de novo synthesis of iron-sulfur (Fe-S) clusters within mitochondria, which is required for maturation of both mitochondrial and cytoplasmic [2Fe-2S] and [4Fe-4S] proteins.First, a [2Fe-2S] cluster is transiently assembled on the scaffold protein ISCU. In a second step, the cluster is released from ISCU, transferred to a glutaredoxin GLRX5, followed by the formation of mitochondrial [2Fe-2S] proteins, the synthesis of [4Fe-4S] clusters and their target-specific insertion into the recipient apoproteins. Cluster assembly on ISCU depends on the function of the cysteine desulfurase complex NFS1-LYRM4/ISD11, which serves as the sulfur donor for cluster synthesis, the iron-binding protein frataxin as the putative iron donor, and the electron transfer chain comprised of ferredoxin reductase and ferredoxin, which receive their electrons from NADH (By similarity).

Product :Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Recognizes endogenous levels of ISCU protein

BiowMW :~ 17 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :NIFUN; Iron-sulfur cluster assembly enzyme ISCU mitochondrial; NifU-like N-terminal domain-containing protein; NifU-like protein

Immunogen :Recombinant fusion protein of human ISCU. The exact sequence is proprietary.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of ISCU expression in HEK293T (A), A549 (B) whole cell lysates.

免疫沉淀（IP）

免疫组织化学（IHC）

免疫荧光（IF）