产品类别

联系我们

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

BCKDHA polyclonal antibody

货号: BS67347

价格：￥ 1140

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS67347

Product Name :BCKDHA polyclonal antibody

Swiss-Prot :P12694

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IF/ICC

Application_all :WB (1/500 - 1/2000), IF/ICC (1/50 - 1/200)

Background :Branched-chain amino acids (BCAAs) leucine, isoleucine, and valine are essential amino acids in mammals, but elevated levels of BCAAs have been implicated in cardiovascular and metabolic disorders. The branched-chain α-keto acid dehydrogenase complex (BCKDH) catalyzes the rate-limiting step in the BCAA degradation pathway. Branched-chain α-keto acid decarboxylase (BCKDH-E1) is one of three enzymatic components in this complex. The α subunit of BCKDH-E1 (BCKDH-E1α) is critical for the regulation of BCKDH. Phosphorylation of BCKDH-E1α was shown to play a key role in regulating the enzymatic activity of this complex. Phosphorylation of BCKDH-E1α at Ser293 inactivates BCKDH. A significant elevation in plasma BCAA levels was reported to correlate with increased phosphorylation of BCKDH-E1α at Ser293 and suppressed BCKDH activity in the liver of diabetic mice.

Product :Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Recognizes endogenous levels of BCKDHA protein.

BiowMW :~50 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :2-oxoisovalerate dehydrogenase subunit alpha mitochondrial; Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain; BCKDE1A; BCKDH E1-alpha

Immunogen :Recombinant full length protein of human BCKDHA

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of BCKDHA expression in LO2 (A), NIH3T3 (B), mouse heart (C), rat liver (D) whole cell lysates.