产品类别

联系我们

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

His-tag polyclonal antibody

货号: BS67270

价格：￥ 1140

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS67270

Product Name :His-tag polyclonal antibody

Swiss-Prot :N/A

Host :Rabbit

Reactivity :N/A

Applications :WB, IP

Application_all :WB (1/1000 - 1/2000), IP (1/10 - 1/100)

Background :The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. A system that has proven to be very successful relies on the insertion of a six histidine (His6) sequence in the N-terminus of the encoded protein, allowing for efficient coupling to Ni2+-chelating resins and purification by single step affinity chromatography. This polyhistidine sequence can then be removed by specific cleavage at sites recognized by enzymes such as thrombin or enterokinase, permitting the separation of the target protein from the polyhistidine tag.

Product :Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.

BiowMW :N/A

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; His tag

Immunogen :KLH-conjugated synthetic peptide HHHHHH.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of Anti-His-tag Antibody against HEK293T cells transfected with vector overexpressing His tag (A) and untransfected (B).

免疫沉淀（IP）

免疫组织化学（IHC）

免疫荧光（IF）