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Histone H3 (MonoMethyl-K4) polyclonal antibody

货号: BS67132

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS67132

Product Name :Histone H3 (MonoMethyl-K4) polyclonal antibody

Swiss-Prot :P68431; Q71DI3; P84243; Q6NXT2

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC

Application_all :WB (1/500 - 1/1000), IH (1/50 - 1/200)

Background :Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Product :Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Recognizes endogenous levels of Histone H3 with a site at MonoMethyl-K4 protein.

BiowMW :~ 17 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :HIST1H3A; H3FA; HIST1H3B; H3FL; HIST1H3C; H3FC; HIST1H3D; H3FB; HIST1H3E; H3FD; HIST1H3F; H3FI; HIST1H3G; H3FH; HIST1H3H; H3FK; HIST1H3I; H3FF; HIST1H3J; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; HIST2H3A; HIST2H3C; H3F2; H3FM; HIST2H3D; Histone H3.2; Histone H3/m; Histone H3/o; H3F3A; H3.3A; H3F3; PP781; H3F3B; H3.3B; Histone H3.3; H3F3C; Histone H3.3C; Histone H3.5

Immunogen :KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Histone H3 with a site at MonoMethyl-K4. The exact sequence is proprietary.

Conjugate :Unconjugated

Modification :Methylated

Western blot analysis of Histone H3 (MonoMethyl-K4) expression in MCF7 (A), U2OS (B), DLD (C), rat lung (D), rat kidney (E) whole cell lysates.

Immunohistochemical analysis of Histone H3 (MonoMethyl-K4) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.