Western blot analysis of NPDC1 expression in HEK293T (A), H1792 (B), PC12 (C), mouse lung (D), mouse heart (E), rat testis (F), rat heart (G) whole cell lysates.
Immunohistochemical analysis of NPDC1 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.