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PARD3 polyclonal antibody

货号: BS67021

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS67021

Product Name :PARD3 polyclonal antibody

Swiss-Prot :Q8TEW0

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC

Application_all :WB (1/500 - 1/1000), IH (1/100 - 1/200)

Background :Adapter protein involved in asymmetrical cell division and cell polarization processes (PubMed:27925688, PubMed:10954424).Seems to play a central role in the formation of epithelial tight junctions (PubMed:27925688).

Product :Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Recognizes endogenous levels of PARD3 protein.

BiowMW :~ 180 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :PAR3; PAR3A; Partitioning defective 3 homolog; PAR-3; PARD-3; Atypical PKC isotype-specific-interacting protein; ASIP; CTCL tumor antigen se2-5; PAR3-alpha

Immunogen :KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human PARD3. The exact sequence is proprietary.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of PARD3 expression in HEK293T (A), U2OS (B), A549 (C) whole cell lysates.

Immunohistochemical analysis of PARD3 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.