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Monoclonal Anti-MMAE specific Antibody, Rabbit IgG (M1D12)

货号: BS66200

价格：￥

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS66200

Product Name :Monoclonal Anti-MMAE specific Antibody, Rabbit IgG (M1D12)

Host :Rabbit

Applications :ELISA

Application_all :ELISA 0.06-125 ng/mL

Background :Monomethyl auristatin E (MMAE) is a synthetic derivative of dolastatin 10 and functions as a potent mitotic inhibitor by inhibiting tubulin polymerization. MMAE is widely used as a cytotoxic component of antibody-drug conjugates (ADCs) to treat several cancer types. Anti-MMAE antibody is a rabbit monoclonal antibody specially react with MMAE without MMAF, which is more sensitive than mouse antibody. The anti-MMAE antibody is a useful reagent in PK assay to determine conjugated antibodies.

Product :Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Purification&Purity :Protein A purified / Protein G purified

Storage&Stability :-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Specificity :Specifically recognizes MMAE and does not recognize MMAF.

Note :For research use only, not for use in diagnostic procedure.

Immunogen :MMAE-BSA

Conjugate :Unconjugated

Modification :Unmodified

Immobilized Disitamab Vedotin (RC48) at 0.2 μg/mL (100 μL/well) can bind Monoclonal Anti-MMAE specific Antibody, Rabbit IgG (M1D12) with a linear range of 0.06-2 ng/mL (QC tested).

Immobilized Monoclonal Anti-MMAE specific Antibody, Rabbit IgG (M1D12) at 5 μg/mL, add Disitamab Vedotin (RC48) in the 100% Human Serum and then add Biotinylated Human Her2, His,Avitag, premium grade at 0.5 μg/mL. Detection was performed using HRP-conjugated Streptavidin (QC tested).

Immobilized Monoclonal Anti-MMAE specific Antibody, Rabbit IgG (M1D12) at 5 μg/mL, add Disitamab Vedotin (RC48) and then add Biotinylated Human Her2, His,Avitag, premium grade at 0.5 μg/mL. Detection was performed using HRP-conjugated Streptavidin (Routinely tested).

Serial dilutions of Monoclonal Anti-MMAE specific Antibody, Rabbit IgG (M1D12): Disitamab Vedotin (RC48) binding reactions. The half maximal inhibitory concentration (IC50) is 0.006187 μg/mL (Routinely tested).