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ox-LDL monoclonal antibody

货号: BS66182

价格：￥ 1350

货期：现货（1-2个工作日）

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详细信息检测数据实验指南文献

No. :BS66182

Product Name :ox-LDL monoclonal antibody

Host :Mouse

Reactivity :Mouse,Rat

Applications :WB IF/ICC IHC ELISA

Application_all :WB 1:500 - 1:2000 IHC 1:100 - 1:500 IF/ICC 1:100 - 1:200 ELISA 1:5000 - 1:10000

Background :Low-density lipoprotein (LDL) is the carrier protein for cholesterol in the blood. LDL binds to its receptor on the capillary walls and thereby mediates the uptake and clearence of cholesterol from the circulation. In atherosclerotic lesions oxidatively modified LDL is found and oxidized LDL is specifically recognized and ingested by macrophages via scavenger receptor A and CD36. Oxidized LDL may be a marker of atherosclerosis but the precise changes in oxidized LDL are not well described. Low-density lipoprotein oxidised with Cu2SO4.

Product :0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :ox-LDL monoclonal antibody detects endogenous levels of ox-LDL protein.

BiowMW :~ 31 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :ox-LDL: LDL (Copper oxidized); Cu2SO4 oxidized low density lipoprotein; oxidized low density lipoprotein; Low density lipoprotein; MDA oxidized LDL; MDA oxidized low density lipoprotein

Immunogen :Full length protein from human plasma.

Conjugate :Unconjugated

Modification :Unmodified

Paraformaldehyde-fixed, paraffin embedded (Mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ox-LDL) Monoclonal Antibody, Unconjugated at 1:2000 overnight at 4°C.

Paraformaldehyde-fixed, paraffin embedded (rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (ox-LDL) Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C.