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BDNF Polyclonal Antibody

货号: BS66135

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS66135

Product Name :BDNF Polyclonal Antibody

Swiss-Prot :P23560

Host :Rabbit

Reactivity :Human,Mouse,Rat,

Applications :WB IF/ICC

Application_all :WB: 1:500-1:2000 IF/ICC: 1:50-1:200

Background :During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.

Product :0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :BDNF Polyclonal Antibody detects endogenous levels of BDNF protein.

BiowMW :~ 25 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Abrineurin; Brain Derived Neurotrophic Factor; MGC34632; Neurotrophin; BDNF_HUMAN

Immunogen :KLH conjugated synthetic peptide derived from human BDNF: 81-180/247

Conjugate :Unconjugated

Modification :Unmodification

Sample: Brain(Rat)lysate at 30ug;Primary: Anti-BDNF at 1:200

Tissue/cell: rabbit brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37℃ for 20 min;Incubation: Anti-BDNF Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C.

Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37℃ for 20 min;Incubation: Anti-BDNF Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C.