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Rictor (Phospho-Thr1135) polyclonal antibody（KU）

货号: BS65995

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :BS65995

Product Name :Rictor (Phospho-Thr1135) polyclonal antibody（KU）

Swiss-Prot :Q6R327

Host :Rabbit

Reactivity :Human

Applications :WB IF IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Background :Cell growth is a fundamental biological process whereby cells accumulate mass and increase in size. The mammalian TOR (mTOR) pathway regulates growth by coordinating energy and nutrient signals with growth factor-derived signals. mTOR is a large protein kinase with two different complexes. One complex contains mTOR, GβL and raptor, which is a target of rapamycin. The other complex, insensitive to rapamycin, includes mTOR, GβL, Sin1, and rictor. The mTOR-rictor complex phosphorylates Ser473 of Akt/PKB in vitro. This phosphorylation is essential for full Akt/PKB activation. Furthermore, an siRNA knockdown of rictor inhibits Ser473 phosphorylation in 3T3-L1 adipocytes. This complex has also been shown to phosphorylate the rapamycin-resistant mutants of S6K1, another effector of mTOR. Phosphorylation of Thr1135 on rictor was identified at Cell Signaling Technology (CST) using PhosphoScan®, CST's LC-MS/MS platform for phosphorylation site discovery. Additional research indicates that rictor is phosphorylated at Thr1135 by p70 S6K, which negatively regulates mTORC2 protein complex as part of a negative feedback mechanism controlling Akt activity.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Rictor (Phospho-Thr1135) polyclonal antibody detects endogenous levels of Rictor protein only when phosphorylated at Thr1135.

BiowMW :~ 210 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :PI3K AKT signaling,Translational Contral elF4 and p70 S6 Kinase,MTOR signaling,

Alternative Name :Rapamycin-insensitive companion of mTOR; AVO3 homolog; hAVO3; RICTOR; KIAA1999

Immunogen :Synthetic phosphopeptide derived from human Rictor around the phosphorylation site of Threonine 1135.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of Rictor (Phospho-Thr1135) polyclonal antibody at 1:500 dilution Lane1:Hela treated with λ-phosphatase whole cell lysate(40ug) Lane2:HeLa treated with IGF-1(50 ng/ml,30 minutes) whole cell lysate(40ug) Lane3:HeLa treated with IGF-1(50 ng/ml,15 minutes) whole cell lysate(40ug) Lane4:HeLa whole cell lysate(40ug)

Immunofluorescence analysis of Hela cells using Rictor antibody at dilution of 1:50.