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Ankyrin erythroid Polyclonal Antibody

货号: BS65980

价格：￥ ——

货期：现货（1-2个工作日）

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No. :BS65980

Product Name :Ankyrin erythroid Polyclonal Antibody

Swiss-Prot :P16157

Host :Rabbit

Reactivity :Human,Mouse,Rat,Dog,Pig,Cow,Horse,

Applications :IHC-P,

Application_all :IHC-P=1:100-500

Background :Ankyrins are a family of proteins that link the integral membrane proteins to the underlying spectrin-actin cytoskeleton and play key roles in activities such as cell motility, activation, proliferation, contact and the maintenance of specialized membrane domains. Multiple isoforms of ankyrin with different affinities for various target proteins are expressed in a tissue-specific, developmentally regulated manner. Most ankyrins are typically composed of three structural domains: an amino-terminal domain containing multiple ankyrin repeats; a central region with a highly conserved spectrin binding domain; and a carboxy-terminal regulatory domain which is the least conserved and subject to variation. Ankyrin 1, the prototype of this family, was first discovered in the erythrocytes, but since has also been found in brain and muscles. Mutations in erythrocytic ankyrin 1 have been associated in approximately half of all patients with hereditary spherocytosis. Complex patterns of alternative splicing in the regulatory domain, giving rise to different isoforms of ankyrin 1 have been described. Truncated muscle-specific isoforms of ankyrin1 resulting from usage of an alternate promoter have also been identified

Product :0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Purification&Purity :affinity purified by Protein A

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Ankyrin erythroid Polyclonal Antibody detects endogenous levels of Ankyrin erythroid protein.

BiowMW :206kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :ANK; ANK-1; Ank1; ANK1_HUMAN; Ankyrin erythroid; Ankyrin 1, erythrocytic; Ankyrin R; Ankyrin-1; Ankyrin-R; Erythrocyte ankyrin; SPH1; SPH2.

Immunogen :KLH conjugated synthetic peptide derived from human Ankyrin erythroid:501-600/1881

Conjugate :Unconjugated

Modification :Unmodification

Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Ankyrin erythroid) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C

Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Ankyrin erythroid) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C