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IKB alpha Polyclonal Antibody

货号: BS65736

价格：￥ 1140

货期：现货（1-2个工作日）

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详细信息检测数据实验指南文献

No. :BS65736

Product Name :IKB alpha Polyclonal Antibody

Swiss-Prot :Q9Z1E3

Host :Rabbit

Reactivity :Human,Mouse,Rat,

Applications :WB,IHC-P,FCM,

Application_all :WB=1:500-2000，IHC-P=1:100-500，Flow-Cyt=1ug/test

Background :Three major forms of IKB like molecules have been identified and each is characterised by multiple copies of ankyrin repeats. IKB alpha and IKB beta appear to be the major regulatory forms of IKB in most cells. These proteins interact with p65 or cRel containing forms of NFkB and block nuclear import by masking the nuclear localisation sequences of NFkB. The activation of NFkB involves the inducible phosphorylation and subsequent degradation of IKB. Immunoblotting easily detects the hyperphosphorylated forms of IKB alpha, but not phosphorylated IKB beta. Interestingly, IKB alpha and IKB beta mediate different NFkB responses. IkB alpha appears to control more transient activation of NFkB in response to an inducer, while IKB beta controls a persistent response. Bcl3 interacts with p50 and p52 containing forms of NFkB, but rather than being an inhibitor it appears to function to stimulate transcription. The degradation of IKB is confirmed by immunoblotting.

Product :0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Purification&Purity :affinity purified by Protein A

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :IKB alpha Polyclonal Antibody detects endogenous levels of IKB alpha protein.

BiowMW :37 kD

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :NFKBIA; Inhibitor of KB alpha; I kappa B alpha; I(Kappa)B(alpha); IkappaBalpha; IKBA; IKBalpha; MAD 3; MAD3; Major histocompatibility complex enhancer binding protein MAD3; NF kappa B inhibitor alpha; NFKBI; Nuclear factor of kappa light chain gene enhancer in B cells; Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha; IKBA_HUMAN.

Immunogen :KLH conjugated synthetic peptide derived from human NFKBIA:1-120/314

Conjugate :Unconjugated

Modification :Unmodification

Primary: Anti-IKB alpha at 1/1000 dilution

Paraformaldehyde-fixed, paraffin embedded (mouse lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IKB alpha) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IKB alpha) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C

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