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YKL-40/CHI3L1 polyclonal antibody

货号: BS6564

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS6564

Product Name :YKL-40/CHI3L1 polyclonal antibody

Swiss-Prot :P36222

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC, IF/ICC

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200|IF/ICC,1:50 - 1:200

Background :Chitinases catalyze the hydrolysis of chitin, which is an abundant glycopolymer found in insect exoskeletons and fungal cell walls. The glycoside hydrolase 18 family of chitinases includes eight human family members. This gene encodes a glycoprotein member of the glycosyl hydrolase 18 family. The protein lacks chitinase activity and is secreted by activated macrophages, chondrocytes, neutrophils and synovial cells. The protein is thought to play a role in the process of inflammation and tissue remodeling.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :30-40KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :CHI3L1;ASRT7;CGP-39;GP-39;GP39;HC-gp39;HCGP-3P;YKL-40;YKL40;YYL-40;hCGP-39

Immunogen :Recombinant fusion protein of human YKL-40/CHI3L1(NP_001267.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of THP-1 cells, using YKL-40/CHI3L1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit .
Exposure time: 180s.

Immunohistochemistry of paraffin-embedded mouse liver using YKL-40/CHI3L1 antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded mouse spleen using YKL-40/CHI3L1 antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunofluorescence analysis of THP-1 cells using YKL-40/CHI3L1 Rabbit pAb at dilution of 1:100 . Blue: DAPI for nuclear staining.

ME2 Promotes Proneural–Mesenchymal Transition and Lipogenesis in Glioblastoma

JournalFrontiers in Oncology

ApplicationWB

IF6.244

SpeciesHuman

PMID34381734
FoxM1 drives ADAM17/EGFR activation loop to promote mesenchymal transition in glioblastoma

JournalCell Death & Disease

ApplicationWB,IF

IF5.959

SpeciesHuman

PMID29700308