Tissue/cell: mouse kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,) at 37℃ for 20 min;
Incubation: Anti-Phospho-FRA1 (Ser265) Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C
Tissue/cell: human gastric cancer; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,) at 37℃ for 20 min;
Incubation: Anti-Phospho-IRAK1 (Ser376) Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C