产品类别

联系我们

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

Caspase 3 precursor Polyclonal Antibody

货号: BS65606

价格：￥ 1140

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS65606

Product Name :Caspase 3 precursor Polyclonal Antibody

Swiss-Prot :P55213

Host :Rabbit

Reactivity :Human, Mouse, Rat, (predicted: Dog, Rabbit, )

Applications :WB，

Application_all :WB=1:500-2000 IHC-P=1:100-500

Background :Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme. Gene for Caspase 3 also known as Yama, CPP32, and apopain codes for a 32-kDa protein. Caspase 3 cleaves the death substrate poly(ADP-ribose) polymerase (PARP) to a specific 85 kDa form observed during apoptosis and is inhibitable by the CrmA protein. Other Caspase 3 substrates include DNA-PK, actin, GAS2, and procaspase-6, etc. Caspase 3 is activated by cleavage events at Asp-28/Ser-29 (between N-terminal pro-domain) and Asp-175/Ser-176 (between large and small subunits) to generate a large subunit of 17-kDa and a small subunit of 12-kDa.

Product :0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Purification&Purity :affinity purified by Protein A

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Caspase 3 Polyclonal Antibody detects endogenous levels of Caspase 3 protein.

BiowMW :28kD

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Caspase 3 precursor; APOPAIN; CASP3; Caspase 3 apoptosis related cysteine protease; Caspase3; CPP32; CPP32B; Cysteine protease CPP32; Human cysteine protease CPP32 isoform alpha mRNA complete cds; PARP cleavage protease; SCA 1; SCA1; SREBP cleavage activity 1; Yama; CASP3_HUMAN; Caspase-3; CASP-3; Apopain; Protein Yama; SREBP cleavage activity 1; SCA-1.

Immunogen :KLH conjugated synthetic peptide derived from human Caspase 3 precursor:11-120/277

Conjugate :Unconjugated

Modification :Unmodification

Sample: Spleen (Mouse) Lysate at 40 ug Primary: Anti-Caspase 3 precursor at 1/1000 dilution

Sample: Lane 1: Spleen (Mouse) Lysate at 40 ug Lane 2: Lung (Mouse) Lysate at 40 ug Primary: Anti-Caspase 3 precursor at 1/1000 dilution

NOAD, a novel nitric oxide donor, induces G2/M phase arrest and apoptosis in human hepatocellular carcinoma Bel-7402 cells

JournalToxicology In Vitro

ApplicationWB

IF3.5

SpeciesHuman

PMID26001517
Up-regulation of Tight-Junction Proteins by p38 Mitogen-Activated Protein Kinase/p53 Inhibition Leads to a Reduction of Injury to the Intestinal Mucosal Barrier in Severe Acute Pancreatitis

JournalPancreas

ApplicationWB

IF3.327

SpeciesRat

PMID27171513
Di(2‐ethylhexyl) phthalate induces apoptosis through mitochondrial pathway in GC‐2spd cells

JournalEnvironmental Toxicology

ApplicationWB

IF3.118

SpeciesMouse

PMID27416487
Di-2-ethylhexyl phthalate induced oxidative damage involving FasL-associated apoptotic pathway in mouse spermatogenic GC-2spd cells

JournalMolecular & Cellular Toxicology

ApplicationWB

IF1.83

SpeciesMouse

PMID
Arsenic and sulfur dioxide co-exposure induce renal injury via activation of the NF-κB and caspase signaling pathway

JournalChemosphere

ApplicationWB

IF7.086

SpeciesHuman

PMID30825854