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E2F3 polyclonal antibody

货号: BS65526

价格：￥ ——

货期：现货（1-2个工作日）

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No. :BS65526

Product Name :E2F3 polyclonal antibody

Swiss-Prot :O00716

Host :Rabbit

Reactivity :Human,Mouse,Rat,Cow,Horse,Rabbit,Sheep,

Applications :IHC-P,

Application_all :IHC-P=1:100-500（石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.

Background :The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F1 and E2F2, have an additional cyclin binding domain. This protein binds specifically to retinoblastoma protein pRB in a cell-cycle dependent manner. [provided by RefSeq].

Product :0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Purification&Purity :affinity purified by Protein A

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :E2F3 polyclonal Antibody detects endogenous levels of E2F3 protein.

BiowMW :~50kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :DKFZp686C18211; E2F 3; E2F transcription factor 3; KIAA0075; MGC104598; Transcription factor E2F 3; E2F3_HUMAN; Transcription factor E2F3; E2F-3.

Immunogen :KLH conjugated synthetic peptide derived from human E2F3:91-190/465

Conjugate :Unconjugated

Modification :Unmodification

Paraformaldehyde-fixed, paraffin embedded (rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (E2F3) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C.

Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (E2F3) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C.

Paraformaldehyde-fixed, paraffin embedded (mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (E2F3) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C.